Officer Ray Tensing, of the University of Cincinnati police department, is shown in this Greenhills Police Department photo released on July 29, 2015.
DuBose's death fueled demonstrations against use of lethal force by white officers against unarmed blacks and other minorities, an issue that renewed debate over racial bias in USA police departments.
He is also charged with voluntary manslaughter.
Judge Leslie Ghiz expects the prosecution will call UC police officers Lt. Tim Barge, Philip Kidd and David Lindenschmidt, and a civilian witness.
Opening arguments are set for Thursday morning at a county courthouse in Cincinnati. Many, but not all, say they have previously seen at least parts the body camera video from the incident. She said he disregarded his police training during the traffic stop. Mathews said the defense would remain unchanged. The vehicle started rolling forward slowly, and with his arm pinned against the steering wheel, according to his lawyer, Tensing pulled his gun and fired once.
By comparison, the first trial jury consisted of six white women, four white men, and two African-American women.
Among those excused for cause were a black man who said officers unfairly target blacks and a white man who wrote in his questionnaire that Tensing should get a medal.
Hamilton County Prosecutor Joe Deters said last fall he felt his team had proven murder.